pCYP05
(Plasmid
#212838)
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PurposeExpresses the Burkholderia multivorans LRD-modifying cytochrome P450 enzyme
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 212838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCYP
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecytochrome P450
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Alt nameUniprot ID: A0A0H3KUT7
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SpeciesBurkholderia multivorans
- Promoter PcymRC
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bycodon optimized and synthesized by vendors
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCYP05 was a gift from Christopher Voigt (Addgene plasmid # 212838 ; http://n2t.net/addgene:212838 ; RRID:Addgene_212838) -
For your References section:
Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s. Lin GM, Voigt CA. Nature Catalysis (2023) 10.1038/s41929-023-01049-5
