eGFP-SUN1ΔN
(Plasmid #213508)

• Purpose: Expresses human eGFP-SUNΔN in mammalian cells
• Depositing Lab: Karim Mekhail
• Depositing Organization: University of Toronto
  Located in Canada
• Publication: Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319-1330. doi: 10.1038/s41594-024-01286-7. Epub 2024 Apr 17.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 8428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP-SUNΔN
• Species: H. sapiens (human)
• Mutation: SUN1ΔN has the first 138 amino acid (lamina domain) of the WT removed. It contains a silent mutation of SUN1ΔN's 861th nucleotide (C->T). It confers resistance to a siSUN1 with a target sequence of CCGTGTTGAATTGGGCAAGCA.
• GenBank ID: AB648918.1
• Entrez Gene: SUN1 (a.k.a. UNC84A)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CGC AAA TGG GCG GTA GGC GTG
• 3′ sequencing primer: TAG AAG GCA CAG TCG AGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see https://www.biorxiv.org/content/10.1101/2023.05.07.539750v1 for bioRxiv preprint

DNA (Catalog # 213508-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 213508-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.