PKD1-F (pCI-PKD1-Flag)
(Plasmid #21369)

• Depositing Lab: Gregory Germino
• Depositing Organization: Johns Hopkins University and School of Medicine
  Located in the United States of America
• Publication: Hanaoka et al Nature. . 408(6815):990-4.
• Citations: 3 Articles

Backbone

• Vector backbone: pCI (modified)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4713
• Vector type: Mammalian Expression
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a in dYT at 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: autosomal dominant polycystic kidney disease type I
• Alt name: PKD1 wild-type
• Species: H. sapiens (human)
• Insert Size (bp): 13117
• Mutation: wild-type sequence*
• GenBank ID: L33243
• Entrez Gene: PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note that the PKD in this plasmid does have a T3206C, which changes a serine to a proline. From the depositor: "As far as [we] know, this missense change is not pathologic and the cDNA including this change appears to be functional using several assays but it is not the standard PKD1 sequence in the database."

DNA (Catalog # 21369-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 21369-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.