pIRES_iSeroSnFR_cytosolic
(Plasmid #214132)

• Purpose: Fluorescent biosensor for intracellular serotonin (5-HT)
• Depositing Lab: Steffen Sinning
• Depositing Organization: Aarhus University
  Located in Denmark
• Publication: Bukowski et al Sci Rep. 2024 Mar 19;14(1):6541. doi: 10.1038/s41598-024-56712-z.
• Citations: 1 Article

Backbone

• Vector backbone: pIRES
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6523
• Total vector size (bp): 8134
• Modifications to backbone: Blasticidin resistance gene inserted in cassette B after IRES element for bicistronic expression of blasticidin resistance gene together with iSeroSnFR_cytosolic
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iSeroSnFR_cytosolic
• Species: Synthetic
• Insert Size (bp): 1611
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Elizabeth Unger & Lin Tian

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-3272320/v1 for Research Square preprint.

iSeroSnFR and iSeroSnFR_cytosolic originally described in Unger et al, 2020 (DOI: https://doi.org/10.1016/j.cell.2020.11.040)

DNA (Catalog # 214132-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 214132-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.