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(Plasmid #214235)


Item Catalog # Description Quantity Price (USD)
Plasmid 214235 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3700
  • Total vector size (bp) 4534
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Syk kinase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Sequence gained through ancestral reconstruction of Syk and ZAP-70 kinase domains and codon optimized for E. coli expression
  • Entrez Gene
    SYK (a.k.a. IMD82, p72-Syk)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This protein needs to be co-expressed in E. coli BL21(DE3) cells with the YopH tyrosine phosphatase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-H6-TEV-AncSZ was a gift from Neel Shah (Addgene plasmid # 214235 ; ; RRID:Addgene_214235)
  • For your References section:

    Saturation mutagenesis of a predicted ancestral Syk-family kinase. Hobbs HT, Shah NH, Shoemaker SR, Amacher JF, Marqusee S, Kuriyan J. Protein Sci. 2022 Oct;31(10):e4411. doi: 10.1002/pro.4411. 10.1002/pro.4411 PubMed 36173161