pMK382
(Plasmid
#214373)
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PurposeTet-OsTIR1(F74G) (AAVS1 insertion donor)
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Depositing Lab
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Depositing OrganizationNational Institute of Genetics
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 214373-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 214373-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMK243
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTet-OsTIR1(F74G)
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SpeciesH. sapiens (human), M. musculus (mouse)
- Promoter Tet ON 3G promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Mlu (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer ggaaacagctatgaccatg
- 3′ sequencing primer gtaaaacgacggccagt
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use this donor plasmid with AAVS1 CRISPR T2 (Addgene #72833) for integration at the AAVS1 locus.
DNA (Catalog # 214373-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 214373-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK382 was a gift from Masato Kanemaki (Addgene plasmid # 214373 ; http://n2t.net/addgene:214373 ; RRID:Addgene_214373) -
For your References section:
Combination of AID2 and BromoTag expands the utility of degron-based protein knockdowns. Hatoyama Y, Islam M, Bond AG, Hayashi KI, Ciulli A, Kanemaki MT. EMBO Rep. 2024 Sep;25(9):4062-4077. doi: 10.1038/s44319-024-00224-4. Epub 2024 Aug 23. 10.1038/s44319-024-00224-4 PubMed 39179892
