pMIA4.721-Olônne
(Plasmid
#214443)
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PurposeBxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMIA4.721
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Vector typeHDR donor
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2xFKBP12-Casp9
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SpeciesSynthetic
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Insert Size (bp)1239
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Tag
/ Fusion Protein
- P2A-Bsd (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIA4.721-Olônne was a gift from Roger Foo (Addgene plasmid # 214443 ; http://n2t.net/addgene:214443 ; RRID:Addgene_214443) -
For your References section:
Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells. Autio MI, Motakis E, Perrin A, Bin Amin T, Tiang Z, Do DV, Wang J, Tan JKM, Ding SSL, Tan WX, Lee CJM, Teo AKK, Foo R. Elife. 2024 Jan 2;13:e79592. doi: 10.7554/eLife.79592. 10.7554/eLife.79592 PubMed 38164941
Map uploaded by the depositor.
