Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells.
Autio MI, Motakis E, Perrin A, Bin Amin T, Tiang Z, Do DV, Wang J, Tan JKM, Ding SSL, Tan WX, Lee CJM, Teo AKK, Foo R
Elife. 2024 Jan 2;13:e79592. doi: 10.7554/eLife.79592. PubMed Article
Autio MI, Motakis E, Perrin A, Bin Amin T, Tiang Z, Do DV, Wang J, Tan JKM, Ding SSL, Tan WX, Lee CJM, Teo AKK, Foo R
Elife. 2024 Jan 2;13:e79592. doi: 10.7554/eLife.79592. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214441 | pMIA4.721-Pansio | BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd. |
| 214443 | pMIA4.721-Olônne | BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd. |
| 214444 | pMIA4.721-Keppel | BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd. |
| 214445 | pMIA3-Pansio | Expresses eSpCas9 and gRNA targeting Pansio-1 GSH |
| 214446 | pMIA3-Keppel | Expresses eSpCas9 and gRNA targeting Keppel-19 GSH |
| 214447 | pMIA3-Olônne | Expresses eSpCas9 and gRNA targeting Olônne-18 GSH |
| 214448 | pMIA22 | Expresses BxbI-BP-NLS |
| 214449 | pMIA10.5-Clover | BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line. |
| 214450 | pMIA10.53-Clover | BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line. |
| 214451 | pMIA10.7 | BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line. |