pMBA325
(Plasmid
#214742)
-
PurposeMedium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214742 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonep15A
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameBBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
-
Speciesother
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains an A76T mutation in glmS. This mutation is not known to affect plasmid function.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMBA325 was a gift from Tae Seok Moon (Addgene plasmid # 214742 ; http://n2t.net/addgene:214742 ; RRID:Addgene_214742) -
For your References section:
Engineering E. coli strains using antibiotic-resistance-gene-free plasmids. Amrofell MB, Rengarajan S, Vo ST, Ramirez Tovar ES, LoBello L, Dantas G, Moon TS. Cell Rep Methods. 2023 Dec 18;3(12):100669. doi: 10.1016/j.crmeth.2023.100669. Epub 2023 Dec 11. 10.1016/j.crmeth.2023.100669 PubMed 38086386
