Engineering E. coli strains using antibiotic-resistance-gene-free plasmids.
Amrofell MB, Rengarajan S, Vo ST, Ramirez Tovar ES, LoBello L, Dantas G, Moon TS
Cell Rep Methods. 2023 Dec 18;3(12):100669. doi: 10.1016/j.crmeth.2023.100669. Epub 2023 Dec 11. PubMed Article
Amrofell MB, Rengarajan S, Vo ST, Ramirez Tovar ES, LoBello L, Dantas G, Moon TS
Cell Rep Methods. 2023 Dec 18;3(12):100669. doi: 10.1016/j.crmeth.2023.100669. Epub 2023 Dec 11. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214738 | pSV015 | Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation |
| 214739 | pSV013 | Medium copy thyA-infA plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation |
| 214740 | pSV014 | High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation |
| 214741 | pMBA324 | Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation |
| 214742 | pMBA325 | Medium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation |
| 214743 | pMBA326 | High copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation |
| 214745 | pMBA333 | Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG) |
| 214746 | pMBA334 | High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG) |
| 214750 | pMBA337 | Medium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validation |
| 214751 | pMBA323 | To create delta(glmS,nadE) working strains and maintaining their viability |