SPYtag-GFPnanobody
(Plasmid
#214837)
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PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepQE80
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSPYtag-GFPnanobody
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SpeciesSynthetic
- Promoter T5
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Tag
/ Fusion Protein
- His-TEV-SUMO- (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CATCAGACAGGCGGTGCGCGTGGTGTCCCGCACATCGTTATGG
- 3′ sequencing primer TAATTAAGCTTACTAACTAACGGTAACTTGCGTGCCTTGA (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SPYtag-GFPnanobody was a gift from Hironori Funabiki (Addgene plasmid # 214837 ; http://n2t.net/addgene:214837 ; RRID:Addgene_214837) -
For your References section:
Cryo-EM analysis on magnetic beads for scarce macromolecules in heterogeneous samples. Arimura Y, Konishi HA, Funabiki H. bioRxiv. 2024. 10.1101/2024.01.21.576499