pME-zfCreERT2
(Plasmid
#214940)
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PurposeGateway middle entry containing zebrafish codon-optimized tamoxifen inducible Cre recombinase
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDONR221
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namezebrafish codon-optimized CreERT2
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Not Specified
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-zfCreERT2 was a gift from Nathan Lawson (Addgene plasmid # 214940 ; http://n2t.net/addgene:214940 ; RRID:Addgene_214940) -
For your References section:
Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafish. Shin M, Yin HM, Shih YH, Nozaki T, Portman D, Toles B, Kolb A, Luk K, Isogai S, Ishida K, Hanasaka T, Parsons MJ, Wolfe SA, Burns CE, Burns CG, Lawson ND. Dev Cell. 2023 Nov 20;58(22):2614-2626.e7. doi: 10.1016/j.devcel.2023.07.022. Epub 2023 Aug 25. 10.1016/j.devcel.2023.07.022 PubMed 37633272