|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21564||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBruce Mayer Lab
- Backbone size w/o insert (bp) 6100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemitogen-activated protein kinase kinase 4
SpeciesM. musculus (mouse)
MutationLysine 129 to arginine
Entrez GeneMap2k4 (a.k.a. JNKK1, MAPKK 4, MEK4, MKK4, PRKMK4, Sek1, Serk1)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
lysine mutant, inactive
missing first 10 amino acids
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEBG-MKK4-K129R (GST) was a gift from Leonard Zon (Addgene plasmid # 21564 ; http://n2t.net/addgene:21564 ; RRID:Addgene_21564)
For your References section:Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun. Sanchez I, Hughes RT, Mayer BJ, Yee K, Woodgett JR, Avruch J, Kyriakis JM, Zon LI. Nature. . 372(6508):794-8. 10.1038/372794a0 PubMed 7997269
Map uploaded by the depositor.