pEBG-MKK4-S255D/T259E (GST)
(Plasmid
#21566)
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Depositing Lab
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Depositing OrganizationBoston Children's Hospital
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 21566-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 21566-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEBG
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Backbone manufacturerBruce Mayer Lab
- Backbone size w/o insert (bp) 6100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemitogen-activated protein kinase kinase 4
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Alt nameMKK4
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Alt nameSEK1
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SpeciesM. musculus (mouse)
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MutationSerine 255 to Aspartic Acid, Threonine 259 to Glutamic Acid
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Entrez GeneMap2k4 (a.k.a. JNKK1, MAPKK 4, MEK4, MKK4, PRKMK4, Sek1, Serk1)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX 5'
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
phosphoacceptor site mutant,
constitutively active,
missing first 10 amino acids
DNA (Catalog # 21566-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 21566-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBG-MKK4-S255D/T259E (GST) was a gift from Leonard Zon (Addgene plasmid # 21566 ; http://n2t.net/addgene:21566 ; RRID:Addgene_21566) -
For your References section:
Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun. Sanchez I, Hughes RT, Mayer BJ, Yee K, Woodgett JR, Avruch J, Kyriakis JM, Zon LI. Nature. . 372(6508):794-8. 10.1038/372794a0 PubMed 7997269
Map uploaded by the depositor.
