|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21604||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK(-)
- Backbone size w/o insert (bp) 3415
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameUAS-hsp70 luciferase
Insert Size (bp)1977
- Cloning method Restriction Enzyme
- 5′ sequencing primer TCCCAGTCACGACGTTGTAAAACGACGGCC
- 3′ sequencing primer CACACAGGAAACAGCTATGACCATGATTAC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUAS-Luc was a gift from Takahiro Kusakabe (Addgene plasmid # 21604 ; http://n2t.net/addgene:21604 ; RRID:Addgene_21604)
For your References section:Analysis of protein interactions with two-hybrid system in cultured insect cells. Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe T. Anal Biochem. 2009 May 26. ():. 10.1016/j.ab.2009.05.033 PubMed 19481053