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pSIQ-EGFP
(Plasmid #216239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216239 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 216239-D50 50 μg of DNA in Tris buffer $495
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DNA 216239-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSIQ
  • Backbone size w/o insert (bp) 8235
  • Total vector size (bp) 8935
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Alt name
    the original enhanced GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CGTCTCAGCCTCACTTTG
  • 3′ sequencing primer CTTGAATTCGAATCGATG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIQ-EGFP was a gift from Hyunju Ro (Addgene plasmid # 216239 ; http://n2t.net/addgene:216239 ; RRID:Addgene_216239)

  • For your References section:

    All-in-one IQ toggle switches with high versatilities for fine-tuning of transgene expression in mammalian cells and tissues. Hong J, Sohn KC, Park HW, Jeon H, Ju E, Lee JG, Lee JS, Rho J, Hur GM, Ro H. Mol Ther Methods Clin Dev. 2024 Feb 2;32(1):101202. doi: 10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14. 10.1016/j.omtm.2024.101202 PubMed 38374964
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