pET28a-SnoopTag-AffiHER2-SpyTag
(Plasmid
#216280)
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PurposeExpresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 5780
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Vector typeBacterial Expression, Affinity Reagent/ Antibody
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSnoopTag-AffiHER2-SpyTag
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Alt nameAffiHER2-SpyTag
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SpeciesSynthetic
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Insert Size (bp)411
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GenBank IDKU296975
- Promoter T7 promoter
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Tags
/ Fusion Proteins
- His6 (N terminal on insert)
- Thrombin cleavage site (N terminal on insert)
- SnoopTag (N terminal on insert)
- SpyTag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-SnoopTag-AffiHER2-SpyTag was a gift from Mark Howarth (Addgene plasmid # 216280)