Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

PARP2-c098
(Plasmid #216873)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 216873 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNIC-CFP
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Parp-c098 needs 10 mM benzamide inhibitor at all steps in the growth media and on plates. Bacteria will grow more slowly in the presence of inhibitor and will need more time for growth. Good mixing and aeration is important for growth in liquid cultures.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PARP2 catalytic domain (no Regulatory Domain)
  • Species
    H. sapiens (human)
  • Entrez Gene
    PARP2 (a.k.a. ADPRT2, ADPRTL2, ADPRTL3, ARTD2, PARP-2, pADPRT-2)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CFP-TEV site-PARP2 cat (no RD), PARP inhibitor Benzamide (10mM) is required during growth

Plasmid mutates easily and depositor recommends routine sequencing of at least the catalytic domain for each miniprep that you prepare.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PARP2-c098 was a gift from Lari Lehtiö (Addgene plasmid # 216873 ; http://n2t.net/addgene:216873 ; RRID:Addgene_216873)