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pEF-DEST51-GOLPH3
(Plasmid #21690)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21690 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 21690-D50 50 μg of DNA in Tris buffer $495
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DNA 21690-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEF-DEST51
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 897
  • Vector type
    Mammalian Expression ; Invitrogen Gateway Destination vector
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Invitrogen stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Golgi Phosphoprotein 3
  • Alt name
    GOLPH3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    897
  • GenBank ID
    NM_022130
  • Entrez Gene
    GOLPH3 (a.k.a. GOPP1, GPP34, MIDAS, Vps74)
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site att (not destroyed)
  • 3′ cloning site att (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-DEST51-GOLPH3 was a gift from Lynda Chin (Addgene plasmid # 21690 ; http://n2t.net/addgene:21690 ; RRID:Addgene_21690)

  • For your References section:

    GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer. Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L. Nature. 2009 Jun 25. 459(7250):1085-90. 10.1038/nature08109 PubMed 19553991
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