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MAP4K3-GFP
(Plasmid #21731)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21731 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/CT-GFP-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6157
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAP4K3
  • Alt name
    GLK
  • Alt name
    MAPKKKK3
  • Alt name
    RAB8IPL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2619
  • GenBank ID
    BC071579
  • Entrez Gene
    MAP4K3 (a.k.a. GLK, MAPKKKK3, MEKKK 3, MEKKK3, RAB8IPL1)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site T/A cloning (unknown if destroyed)
  • 3′ cloning site T/A cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer GFP Reverse (5 ́-GGGTAAGCTTTCCGTATGTAGC-3 ́)
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAP4K3-GFP was a gift from L. Miguel Martins (Addgene plasmid # 21731 ; http://n2t.net/addgene:21731 ; RRID:Addgene_21731)
  • For your References section:

    MAP4K3 modulates cell death via the post-transcriptional regulation of BH3-only proteins. Lam D, Dickens D, Reid EB, Loh SH, Moisoi N, Martins LM. Proc Natl Acad Sci U S A. 2009 Jul 8. ():. 10.1073/pnas.0900608106 PubMed 19587239