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GW1CMV-Perceval
(Plasmid #21737)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21737 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 21737-D50 50 μg of DNA in Tris buffer $495
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DNA 21737-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGW1-CMV
  • Backbone manufacturer
    British Biotech
  • Backbone size w/o insert (bp) 5076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Perceval (fluorescent ATP:ADP sensor)
  • Species
    Synthetic
  • Insert Size (bp)
    1934
  • Mutation
    improved Kozak sequence
  • Tag / Fusion Protein
    • 5'UTR (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-N (rev) gives 5' third
  • 3′ sequencing primer EGFP-C (fwd) gives middle third
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GW1CMV-Perceval was a gift from Gary Yellen (Addgene plasmid # 21737 ; http://n2t.net/addgene:21737 ; RRID:Addgene_21737)

  • For your References section:

    A genetically encoded fluorescent reporter of ATP:ADP ratio. Berg J, Hung YP, Yellen G. Nat Methods. 2009 Feb;6(2):161-6. doi: 10.1038/nmeth.1288. 10.1038/nmeth.1288 PubMed 19122669
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