|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21749||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4969
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsDH5alpha BL21
Copy numberHigh Copy
Alt nameretinoblastoma protein
SpeciesH. sapiens (human)
Insert Size (bp)1670
Mutationdeletion mutant ORF contains aa 379-928 of wt Rb cDNA, contains silent mutation at codon 455 (full-length Rb aa V833 (GTA to GTG)), contains mutation at codon 380 (full-length Rb aa S758L (TCG to TTG))
Entrez GeneRB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, pRb, pp110)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH I (not destroyed)
- 3′ cloning site BamH I (not destroyed)
- 5′ sequencing primer pGex-5'
- 3′ sequencing primer pGex-3' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Rb-379-928-pGex2TK was a gift from William Kaelin (Addgene plasmid # 21749 ; http://n2t.net/addgene:21749 ; RRID:Addgene_21749)
For your References section:Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. Kaelin WG, Krek W, Sellers WR, DeCaprio JA, Ajchenbaum F, Fuchs CS, Chittenden T, Li Y, Farnham PJ, Blanar MA. Cell. 1992 Jul 24. 70(2):351-64. 10.1016/0092-8674(92)90108-O PubMed 1638635