|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21933||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4948
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsDH5alpha BL21
Copy numberHigh Copy
Alt nameretinoblastoma protein
SpeciesH. sapiens (human)
Insert Size (bp)1560
Mutationdeletion mutant ORF contains aa 379-928D703-737 of wt Rb cDNA, contains deletion of aa 703-737 (equaling exon 21), contains a silent mutation at codon 486 (full-length Rb aa Q899 (CAG to CAA))
Entrez GeneRB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH I (not destroyed)
- 3′ cloning site BamH I (not destroyed)
- 5′ sequencing primer pGex-5'
- 3′ sequencing primer pGex-3' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Rb-379-928Δ703-737-pGex2T was a gift from William Kaelin (Addgene plasmid # 21933 ; http://n2t.net/addgene:21933 ; RRID:Addgene_21933)
For your References section:Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product. Kaelin WG, Pallas DC, DeCaprio JA, Kaye FJ, Livingston DM. Cell. 1991 Feb 8. 64(3):521-32. 10.1016/0092-8674(91)90236-R PubMed 1825028