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Rb-379-928Δ738-775-pGex2T
(Plasmid #21783)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21783 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGex2T
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha BL21
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rb
  • Alt name
    retinoblastoma protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1556
  • Mutation
    deletion mutant ORF contains aa 379-928D737-774 of wt Rb cDNA, contains deletion of aa 738-775 (equaling exon 22) contains mutation at codon 154 (full-length Rb aa I532T (ATC to ACC))
  • GenBank ID
    BC040540
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, pRb, pp110)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer pGex-5'
  • 3′ sequencing primer pGex-3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rb-379-928Δ738-775-pGex2T was a gift from William Kaelin (Addgene plasmid # 21783 ; http://n2t.net/addgene:21783 ; RRID:Addgene_21783)
  • For your References section:

    Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product. Kaelin WG, Pallas DC, DeCaprio JA, Kaye FJ, Livingston DM. Cell. 1991 Feb 8. 64(3):521-32. 10.1016/0092-8674(91)90236-R PubMed 1825028