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Rb379-928/C706F-pGex2TK
(Plasmid #21750)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21750 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGex2TK
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha BL21
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rb
  • Alt name
    retinoblastoma protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1670
  • Mutation
    deletion mutant ORF contains aa 379-928 of wt Rb cDNA, contains silent mutation at codon 420 (full-length Rb aa R798 (CGG to CGA) introducing an additional EcoR I-site), contains mutation at codon 328 (full-length Rb aa C706F (TCG to TTG) corresponding to a naturally occurring,loss-of-function RB point mutation known to inactivate pocket binding function [Kaye et al., 1990; Kaelin et al., 1991)
  • GenBank ID
    BC040540
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, pRb, pp110)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer pGex-5'
  • 3′ sequencing primer pGex-3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rb379-928/C706F-pGex2TK was a gift from William Kaelin (Addgene plasmid # 21750 ; http://n2t.net/addgene:21750 ; RRID:Addgene_21750)
  • For your References section:

    Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. Kaelin WG, Krek W, Sellers WR, DeCaprio JA, Ajchenbaum F, Fuchs CS, Chittenden T, Li Y, Farnham PJ, Blanar MA. Cell. 1992 Jul 24. 70(2):351-64. 10.1016/0092-8674(92)90108-O PubMed 1638635