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pQE30-DadA-mut
(Plasmid #217575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217575 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pQE31
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DadA-Y17A-Y18A
  • Species
    Synthetic
  • Insert Size (bp)
    1296
  • Promoter T5
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE30-DadA-mut was a gift from Vsevolod Belousov (Addgene plasmid # 217575 ; http://n2t.net/addgene:217575 ; RRID:Addgene_217575)

  • For your References section:

    Chemogenetic activation of mitochondria counteracts the Warburg effect in tumor cells. Bass DY, Potekhina ES, Ivanenko AV, Moshchenko AA, Korzhenevskiy DA, Nesterenko AM, Shevchenko EK, Karnaeva AE, Zakirova NF, Ivanov AV, Shimolina LE, Shirmanova MV, Lyang OV, Patsap OI, Kudryashova OM, Gazizova GR, Shagimardanova EI, Mariasina SS, Ivlev VA, Bogeski I, Gusev OA, Belousov VV. Nat Chem Biol. 2026 Oct 22. doi: 10.1038/s41589-026-02350-7. 10.1038/s41589-026-02350-7 PubMed 42918903
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