Chemogenetic activation of mitochondria counteracts the Warburg effect in tumor cells.

Bass DY, Potekhina ES, Ivanenko AV, Moshchenko AA, Korzhenevskiy DA, Nesterenko AM, Shevchenko EK, Karnaeva AE, Zakirova NF, Ivanov AV, Shimolina LE, Shirmanova MV, Lyang OV, Patsap OI, Kudryashova OM, Gazizova GR, Shagimardanova EI, Mariasina SS, Ivlev VA, Bogeski I, Gusev OA, Belousov VV
Nat Chem Biol. 2026 Oct 22. doi: 10.1038/s41589-026-02350-7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
217570	pL-CMV-tdmito-mRuby-DadA	Mammalian expression of mRuby-DadA fuse targeted to the mitochondrial matrix
217571	pL-CMV-tdmito-mRuby-DadA-mut	Mammalian expression of inactive mRuby-DadA-mut fuse targeted to the mitochondrial matrix
217572	pL-CMV-tdmito-EGFP-DadA	Mammalian expression of EGFP-DadA fuse targeted to the mitochondrial matrix
217573	pL-CMV-tdmito-EGFP-DadA-mut	Mammalian expression of inactive EGFP=DadA-mut fuse targeted to the mitochondrial matrix
217574	pQE30-DadA	Bacterial expression of DadA
217575	pQE30-DadA-mut	Bacterial expression of inactive DadA mutant
217576	pQE30-mRuby-DadA	Bacterial expression of mRuby-DadA fuse
217577	pQE30-EGFP-DadA	Bacterial expression of EGFP-DadA fuse
217578	pAAV-CAGGS-tdmito-mRuby-DadA	Inserting the gene encoding mRuby-DadA fuse with mitochondrial matrix localization signal into human genome by CRISPR
217579	pAAV-CAGGS-tdmito-mRuby-DadA-mut	Inserting the gene encoding inactive mRuby-DadA-mut fuse with mitochondrial matrix localization signal into human genome by CRISPR
217580	pAAV-CAGGS-FLuc-P2A-tdmito-mRuby-DadA	Inserting the gene encoding mRuby-DadA fuse with mitochondrial matrix localization signal and firefly luciferase into human genome by CRISPR
217581	pAAV-CAGGS-FLuc-P2A-tdmito-mRuby-DadA-mut	Inserting the gene encoding inactive mRuby-DadA-mut with mitochondrial matrix localization signal and firefly luciferase into human genome by CRISPR