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pAAV-CAGGS-FLuc-P2A-tdmito-mRuby-DadA-mut
(Plasmid #217581)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 217581 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    AAV-CAGGS-EGFP
  • Backbone manufacturer
    Addgene (https://www.addgene.org/22212/)
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLuc-P2A-tdmito-mRuby-DadA-Y17A-Y18A
  • Species
    Synthetic
  • Insert Size (bp)
    3933
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The length of the transgene with promoter and 3'-UTR is about 7500 bp, so the plasmid can be used for transfection, but not for AAV production. Please visit https://www.biorxiv.org/content/10.1101/2023.04.18.537329v3 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAGGS-FLuc-P2A-tdmito-mRuby-DadA-mut was a gift from Vsevolod Belousov (Addgene plasmid # 217581 ; http://n2t.net/addgene:217581 ; RRID:Addgene_217581)

  • For your References section:

    Chemogenetic activation of mitochondria counteracts the Warburg effect in tumor cells. Bass DY, Potekhina ES, Ivanenko AV, Moshchenko AA, Korzhenevskiy DA, Nesterenko AM, Shevchenko EK, Karnaeva AE, Zakirova NF, Ivanov AV, Shimolina LE, Shirmanova MV, Lyang OV, Patsap OI, Kudryashova OM, Gazizova GR, Shagimardanova EI, Mariasina SS, Ivlev VA, Bogeski I, Gusev OA, Belousov VV. Nat Chem Biol. 2026 Oct 22. doi: 10.1038/s41589-026-02350-7. 10.1038/s41589-026-02350-7 PubMed 42918903
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