pCW57.1 WT1 +1 ATG
(Plasmid
#217643)
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PurposeDox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)
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Depositing Lab
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Depositing OrganizationUniversity of Birmingham
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217643 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 217643-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 217643-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCW57.1
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Backbone manufacturerAddgene #41393
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWT1 long isoform
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SpeciesH. sapiens (human)
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Entrez GeneWT1 (a.k.a. AWT1, GUD, NPHS4, WAGR, WIT-2, WT-1, WT33)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 217643-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 217643-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCW57.1 WT1 +1 ATG was a gift from Constanze Bonifer (Addgene plasmid # 217643 ; http://n2t.net/addgene:217643 ; RRID:Addgene_217643) -
For your References section:
Isoform-specific and signaling-dependent propagation of acute myeloid leukemia by Wilms tumor 1. Potluri S, Assi SA, Chin PS, Coleman DJL, Pickin A, Moriya S, Seki N, Heidenreich O, Cockerill PN, Bonifer C. Cell Rep. 2021 Apr 20;35(3):109010. doi: 10.1016/j.celrep.2021.109010. 10.1016/j.celrep.2021.109010 PubMed 33882316
