RB-409-815-pGex2T
(Plasmid #21784)

• Depositing Lab: William Kaelin
• Publication: Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Backbone

• Vector backbone: pGex2T
• Backbone manufacturer: Pharmacia
• Backbone size w/o insert (bp): 4948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha BL21
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rb
• Alt name: retinoblastoma protein
• Species: H. sapiens (human)
• Insert Size (bp): 1239
• Mutation: deletion mutant ORF contains aa 409-815 of wt Rb cDNA, contains mutation at codon 341 (full-length Rb aa E748K (GAG to AAG))
• GenBank ID: BC040540
• Entrez Gene: RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: pGex-5'
• 3′ sequencing primer: pGex-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry