pRY1001
(Plasmid #21787)

• Depositing Lab: Richard Yu
• Publication: Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Backbone

• Vector backbone: pACL7-YFP-A206K
• Backbone size w/o insert (bp): 5700
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CFP-Ste12
• Alt name: CFP
• Alt name: Ste12
• Species: S. cerevisiae (budding yeast); A. victoria
• Insert Size (bp): 4254
• Mutation: contains 1000 bp UTR (Ste12 promoter) upstream of CFP-Ste12 ORF contains 3' ADH terminator from pACL7-YFP-A206K.
• GenBank ID: NC_001140
• Entrez Gene: STE12 (a.k.a. YHR084W)
• Tag / Fusion Protein:
  • CFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construction was complicated; please refer to parent article's supplemental materials for details of construction. KpnI site is not unique in this plasmid.