pRY1004
(Plasmid #21788)

• Depositing Lab: Richard Yu
• Publication: Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Backbone

• Vector backbone: pACL7-YFP-A206K
• Backbone size w/o insert (bp): 3300
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dig1-YFP
• Alt name: Dig1
• Alt name: YFP
• Species: S. cerevisiae (budding yeast); A. victoria
• Insert Size (bp): 3500
• Mutation: contains 5' 1000bp UTR (Dig1 promoter) Dig1 open reading frame YFP 3' ADH terminator from pACL7-YFP-A206K
• GenBank ID: NC_001148
• Entrez Gene: DIG1 (a.k.a. YPL049C, RST1)
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construction was complicated; please refer to parent article's supplemental materials for details of construction.