pCL1032
(Plasmid
#217962)
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PurposeMammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.
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Depositing Lab
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Depositing OrganizationUniversity of Warwick
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 217962 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 217962-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 217962-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIRESpuro2
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Backbone manufacturerClonetech
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNASP
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Alt nameNuclear autoantigenic sperm protein
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SpeciesH. sapiens (human)
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Entrez GeneNASP (a.k.a. FLB7527, HMDRA1, PRO1999)
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pEGFP-C2 FP
- 3′ sequencing primer SV40-RP
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 217962-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 217962-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCL1032 was a gift from Andrew Bowman (Addgene plasmid # 217962 ; http://n2t.net/addgene:217962 ; RRID:Addgene_217962) -
For your References section:
Evidence for the nuclear import of histones H3.1 and H4 as monomers. Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ. EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub 2018 Sep 3. 10.15252/embj.201798714 PubMed 30177573
