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Addgene

pToxAmp12
(Plasmid #218286)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 218286 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.30.578080 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pToxAmp12 was a gift from Claudia Vickers (Addgene plasmid # 218286 ; http://n2t.net/addgene:218286 ; RRID:Addgene_218286)
  • For your References section:

    The integration of tandem gene repeats via a bacterial type-II toxin-antitoxin-mediated gene amplification (ToxAmp) system and stability visualisation in Saccharomyces cerevisiae. Evans S, Lu Z, McDonnell L, Anderson W, Peralta F, Watkins T , Ahmed H, Luna-Flores CH, Loan T, Navone L, Trau M, Scott CH, Speight R, Vickers CE, Peng B. bioRxiv 2024.01.30.578080 10.1101/2024.01.30.578080