The integration of tandem gene repeats via a bacterial type-II toxin-antitoxin-mediated gene amplification (ToxAmp) system and stability visualisation in Saccharomyces cerevisiae
Evans S, Lu Z, McDonnell L, Anderson W, Peralta F, Watkins T , Ahmed H, Luna-Flores CH, Loan T, Navone L, Trau M, Scott CH, Speight R, Vickers CE, Peng B
bioRxiv 2024.01.30.578080
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218281 | pJE13HD7 | Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus. |
| 218282 | pITEu2 | Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus |
| 218283 | pITEdv1 | Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus |
| 218284 | pToxamEXP4CUP1 | Use together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locus |
| 218285 | pToxAmp1 | Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism |
| 218286 | pToxAmp12 | Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules |
| 218287 | pToxAmp21R | A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue |