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BiP 2: C. elegans BiP (hsp-4)-GFP
(Plasmid #21896)


Item Catalog # Description Quantity Price (USD)
Plasmid 21896 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Stbl2, 30 degrees
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
  • Mutation
    Fusion between the GFP and hsp-4 takes place at AA#3 of HSP-4
  • Entrez Gene
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This reporter gene tracks the expression of endogenous BiP (hsp-4) in C. elegans. It consists of a ~1.1 KB genomic C. elegans fragment linked to GFP in the vector pPD95.75. The fusion between the GFP artificial gene and hsp-4 takes place at AA #3 of HSP-4)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BiP 2: C. elegans BiP (hsp-4)-GFP was a gift from David Ron (Addgene plasmid # 21896 ; ; RRID:Addgene_21896)
  • For your References section:

    IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Calfon M, Zeng H, Urano F, Till JH, Hubbard SR, Harding HP, Clark SG, Ron D. Nature. 2002 Jan 3. 415(6867):92-6. 10.1038/415092a PubMed 11780124