BiP 2: C. elegans BiP (hsp-4)-GFP
(Plasmid #21896)

• Depositing Lab: David Ron
• Publication: Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Backbone

• Vector backbone: pPD95.75
• Backbone size w/o insert (bp): 4500
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: Stbl2, 30 degrees
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BiP
• Alt name: hsp-4
• Species: C. elegans (nematode)
• Insert Size (bp): 1100
• Mutation: Fusion between the GFP and hsp-4 takes place at AA#3 of HSP-4
• Entrez Gene: hsp-4
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13_pUC-rev
• 3′ sequencing primer: GFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This reporter gene tracks the expression of endogenous BiP (hsp-4) in C. elegans. It consists of a ~1.1 KB genomic C. elegans fragment linked to GFP in the vector pPD95.75. The fusion between the GFP artificial gene and hsp-4 takes place at AA #3 of HSP-4)