Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21929||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1600
Entrez GenePTBP1 (a.k.a. HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
F29L and H62L in Addgene seq result
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-2TK-PTB was a gift from Phil Sharp (Addgene plasmid # 21929 ; http://n2t.net/addgene:21929 ; RRID:Addgene_21929)
For your References section:Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Garcia-Blanco MA, Jamison SF, Sharp PA. Genes Dev. 1989 Dec . 3(12A):1874-86. 10.1101/gad.3.12a.1874 PubMed 2533575