Skip to main content
Addgene
Open Menu

pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2
(Plasmid #219548)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219548 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 219548-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 219548-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pQP-Select
  • Backbone manufacturer
    Originally derived from pEGFP-N1 backbone after several modifications in our lab.
  • Backbone size w/o insert (bp) 3427
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
  • Species
    Synthetic
  • Insert Size (bp)
    3118
  • Mutation
    The (SAGG)x4 linker between GAL4(1-65) and the PCM of IsPadC was replaced with a (SAGG)x1 linker.
  • GenBank ID
    MW890755.1 (part of the insert)
  • Promoter CMVd1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer F1ori-F
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2, NLS-GAL4-Linker fragment was amplified and swapped with NLS-GAL4-4x(SAGG) fragment in pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2 plasmid (Addgene #170273) using NheI and EcoRI restriction sites.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2 was a gift from Vladislav Verkhusha (Addgene plasmid # 219548 ; http://n2t.net/addgene:219548 ; RRID:Addgene_219548)

  • For your References section:

    iLight2: A near-infrared optogenetic tool for gene transcription with low background activation. Baloban M, Kasatkina LA, Verkhusha VV. Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993. 10.1002/pro.4993 PubMed 38647395
Related items:
Commonly requested with: