Skip to main content
Addgene
Open Menu

pInsert-PhoCl-RER
(Plasmid #219676)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 219676 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
Add to Cart
DNA 219676-D50 50 μg of DNA in Tris buffer $495 *
Add to Cart
DNA 219676-D100 100 μg of DNA in Tris buffer $585 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pTREtight2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD4mScarlet-PhoCl-RER
  • Promoter TRE
  • Tag / Fusion Protein
    • mScarlet and PhoCl

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PhoCl2c-RER can be cut out with HindIII and put in the desired vector with
the POI. can be done easily with gibson cloning in one step.

All the plasmids with pTRE vectors have the POI under tetracycline
promoter and hence when transfected needs to be transfected with another
plasmid, rtTA3(Sara knows) in the presence of doxycycline. or it can be
transfected in cells stably expressing the tetracycline-on transactivator
(rtTA3). rtTA3 is the transactivator that binds with doxycycline and sits
at the promoter for initiation of transcription.

While cloning please make sure you include signal peptide in the beginning
if needed. This depends on the specific POI.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInsert-PhoCl-RER was a gift from Helge Ewers (Addgene plasmid # 219676 ; http://n2t.net/addgene:219676 ; RRID:Addgene_219676)

  • For your References section:

    An optogenetic method for the controlled release of single molecules. Kashyap P, Bertelli S, Cao F, Kostritskaia Y, Blank F, Srikanth NA, Schlack-Leigers C, Saleppico R, Bierhuizen D, Lu X, Nickel W, Campbell RE, Plested AJR, Stauber T, Taylor MJ, Ewers H. Nat Methods. 2024 Mar 8. doi: 10.1038/s41592-024-02204-x. 10.1038/s41592-024-02204-x PubMed 38459384
Related items:
Commonly requested with: