dCas9-miniTurbo
(Plasmid
#219822)
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PurposeEF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
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Depositing Lab
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Depositing OrganizationHospital for Sick Children
Located in Canada -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 219822-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 219822-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneCARPID BASU-dCasRx (Addgene #153209)
- Backbone size w/o insert (bp) 10000
- Total vector size (bp) 14999
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Modifications to backboneAddition of dCas9:miniTurbo
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Vector typeMammalian Expression, CRISPR
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesStreptococcus pyogenes
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Insert Size (bp)4000
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MutationD10A/H841A
- Promoter EF1a
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Tag
/ Fusion Protein
- miniTurbo (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer EF1a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 219822-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 219822-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dCas9-miniTurbo was a gift from Philipp Maass (Addgene plasmid # 219822 ; http://n2t.net/addgene:219822 ; RRID:Addgene_219822)
