pmEGFP-C1-Nir3-LNS2
(Plasmid
#220087)
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PurposeC-terminal PA binding domain of Nir3
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Depositing Lab
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Depositing OrganizationUniversity of Pittsburgh (University, School of Medicine, and Cancer Institute)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 220087-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 220087-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1-mEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePITPNM2
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Alt nameNir3
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SpeciesH. sapiens (human)
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Insert Size (bp)855
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Mutationamino acids 925-1209
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Entrez GenePITPNM2 (a.k.a. NIR-3, NIR3, RDGB2, RDGBA2)
- Promoter CMV
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Tag
/ Fusion Protein
- mEGFP-GGGGSHM (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BmtI (not destroyed)
- 3′ cloning site NotI (not destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Shujuan Gao, StonyBrook University, NY
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 220087-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 220087-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmEGFP-C1-Nir3-LNS2 was a gift from Gerry Hammond (Addgene plasmid # 220087 ; http://n2t.net/addgene:220087 ; RRID:Addgene_220087) -
For your References section:
PILS-Nir1 is a sensitive phosphatidic acid biosensor that reveals mechanisms of lipid production. Weckerly CC, Rahn TA, Ehrlich M, Wills RC, Pemberton JG, Airola MV, Hammond GRV. J Cell Biol. 2025 Nov 3;224(11):e202405174. doi: 10.1083/jcb.202405174. Epub 2025 Sep 9. 10.1083/jcb.202405174 PubMed 40923975
