PurposeLentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||166946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3027
Entrez GeneSFPQ (a.k.a. POMP100, PPP1R140, PSF)
- Promoter UbC
/ Fusion Protein
- Halo (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer CMV F
- 3′ sequencing primer M13 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UbC-Halo-SFPQ was a gift from Rosalind Segal (Addgene plasmid # 166946 ; http://n2t.net/addgene:166946 ; RRID:Addgene_166946)
For your References section:Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival. Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH, Walensky LD, Marto JA, Dhe-Paganon S, Segal RA. J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 10.1083/jcb.202005051. 10.1083/jcb.202005051 PubMed 33284322