pCM121
(Plasmid
#220833)
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PurposeEryA sensor with increased sensitivity (mphA and mphR (T17R) mutation) and high level resistance (ermC)
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Depositing Lab
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Depositing OrganizationRice University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 220833-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 220833-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemphR
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MutationT17R
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 220833-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 220833-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM121 was a gift from Matthew Bennett (Addgene plasmid # 220833 ; http://n2t.net/addgene:220833 ; RRID:Addgene_220833) -
For your References section:
Macrolide Biosensor Optimization through Cellular Substrate Sequestration. Miller CA, Ho JM, Parks SE, Bennett MR. ACS Synth Biol. 2021 Feb 19;10(2):258-264. doi: 10.1021/acssynbio.0c00572. Epub 2021 Feb 8. 10.1021/acssynbio.0c00572 PubMed 33555859
Map uploaded by the depositor.
