|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22094||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1900
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The FRT sequence (+strand) is 5'-GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC-3' and the LoxP sequence (+strand) is 5'-ATAACTTCGTATAGCATACATTATACGAAGTTAT-3'. pK11 is missing the polylinker sites between the 5' FRT sequence and the PGKNeo that are present in pM30.
Known unique sites include: SacI, SacII, SalI, BamHI, ApaI, KpnI and HindIII.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pK-11 was a gift from Gail Martin (Addgene plasmid # 22094 ; http://n2t.net/addgene:22094 ; RRID:Addgene_22094)
For your References section:An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Meyers EN, Lewandoski M, Martin GR. Nat Genet. 1998 Feb . 18(2):136-41. 10.1038/ng0298-136 PubMed 9462741
Map uploaded by the depositor.