pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
(Plasmid
#221360)
-
PurposeMammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.
-
Depositing Lab
-
Depositing OrganizationInstitute for Protein Innovation
Located in the United States of America -
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 221360 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 221360-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 221360-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
* Log in to view industry pricing.
Backbone
-
Vector backbonepTipi2.1
-
Backbone manufacturerInstitute for Protein Innovation (IPI)
- Backbone size w/o insert (bp) 3873
-
Vector typeMammalian Expression, Affinity Reagent/ Antibody
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAnti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
-
SpeciesO. cuniculus (rabbit)
-
Insert Size (bp)654
- Promoter CMV
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer WPRE-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that both plasmids, heavy chain (Plasmid 221359) and light chain (Plasmid 221360), are required to make the antibody.
DNA (Catalog # 221360-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 221360-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa was a gift from Institute for Protein Innovation (IPI) (Addgene plasmid # 221360 ; http://n2t.net/addgene:221360 ; RRID:Addgene_221360) -
For your References section:
Evaluating codon optimization strategies for mammalian glycoprotein production with an open-source expression vector. Yang C, Soni R, Visconti SE, Abdollahi M, Belay F, Ghosh A, Duvall SW, Walton CJW, Meijers R, Zhu H. bioRxiv, March 20, 2026 10.64898/2026.03.18.712111
