Skip to main content
Addgene
Open Menu

2MLS-HyperNova/pcDNA3
(Plasmid #221457)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221457 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 221457-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 221457-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
  • Alt name
    2MLS-HN
  • Species
    Synthetic
  • Insert Size (bp)
    900
  • GenBank ID
    LC771173

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that older versions of Snapgene's software may annotate "HyperNova" incorrectly as "SuperNova". We have verified that this plasmid encodes HyperNova. Please see the depositor's annotated file under "Supplemental Information" for additional annotations.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2MLS-HyperNova/pcDNA3 was a gift from Kiwamu Takemoto (Addgene plasmid # 221457 ; http://n2t.net/addgene:221457 ; RRID:Addgene_221457)

  • For your References section:

    Optical inactivation of intracellular molecules by fast-maturating photosensitizing fluorescence protein, HyperNova. Shidara H, Shirai T, Ozaki-Noma R, Jitsuki S, Nagai T, Takemoto K. Commun Biol. 2024 Aug 6;7(1):945. doi: 10.1038/s42003-024-06583-x. 10.1038/s42003-024-06583-x PubMed 39107369
Related items:
Commonly requested with: