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pT7-ABE8e for IVT
(Plasmid #221654)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221654 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pT7
  • Modifications to backbone
    Inactivated T7 promoter to prevent transcription from plasmid
  • Vector type
    CRISPR ; Template for in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABE8e-SpCas9(D10A)
  • Alt name
    SV40_bpNLS-ABE8e Deaminase-SGGSx2-XTENlinker-SGGSx2-SpCas9(D10A)-SGGS-SV40_bpNLS
  • Species
    Synthetic; SpCas9 is from Streptococcus pyogenes
  • Insert Size (bp)
    4815
  • Tags / Fusion Proteins
    • SV40 bpNLS (N terminal on insert)
    • SV40 bpNLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-ABE8e for IVT was a gift from Gregory Newby (Addgene plasmid # 221654 ; http://n2t.net/addgene:221654 ; RRID:Addgene_221654)

  • For your References section:

    In vivo base editing of Angptl3 via lipid nanoparticles to treat cardiovascular disease. Khirallah J, Bloomer H, Wich D, Huang C, Workman JN, Li Y, Newby GA, Liu DR, Xu Q. Mol Ther Nucleic Acids. 2025 Feb 15;36(2):102486. doi: 10.1016/j.omtn.2025.102486. eCollection 2025 Jun 10. 10.1016/j.omtn.2025.102486 PubMed 40129501
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