pcDNA3-HA-Epsin 2
(Plasmid #22241)

• Depositing Lab: Pietro De Camilli
• Publication: Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-65.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Epsin 2
• Alt name: EH domain-binding protein
• Alt name: EHB21
• Species: H. sapiens (human)
• Insert Size (bp): 1900
• GenBank ID: AF062085
• Entrez Gene: EPN2 (a.k.a. EHB21)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry