pGGB_YFP-MiniTurboID
(Plasmid
#222433)
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PurposeGolden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222433 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 222433-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 222433-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 4290
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Vector typeGolden Gate / Green Gate compatible cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMiniTurboID (BirA mutant)
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SpeciesBirA from E. coli, codon optimized for human and mutated
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Insert Size (bp)771
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Mutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, I305V
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Tags
/ Fusion Proteins
- YFP (N terminal on insert)
- Linker (N terminal on insert)
- Linker (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer SP6
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAlice Ting's lab at Stanford University (MiniTurbo cloned from V5-miniTurbo-NES_pCDNA3, Addgene catalog #107170).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 222433-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 222433-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGB_YFP-MiniTurboID was a gift from Dominique Bergmann (Addgene plasmid # 222433 ; http://n2t.net/addgene:222433 ; RRID:Addgene_222433) -
For your References section:
Spatially resolved proteomics of the Arabidopsis stomatal lineage identifies polarity complexes for cell divisions and stomatal pores. Wallner ES, Mair A, Handler D, McWhite C, Xu SL, Dolan L, Bergmann DC. Dev Cell. 2024 May 6;59(9):1096-1109.e5. doi: 10.1016/j.devcel.2024.03.001. Epub 2024 Mar 21. 10.1016/j.devcel.2024.03.001 PubMed 38518768
