L2383-FRB-GPA-NLuc
(Plasmid
#222902)
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PurposePositive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.
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Depositing Lab
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Depositing OrganizationNorthwestern University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 222902-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 222902-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneModified pcDNA3.1(+)
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Backbone manufacturerThermo Fisher
- Backbone size w/o insert (bp) 5370
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRB-GPA-20GS-NanoLuc
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1356
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.12.16.571988 for bioRxiv preprint.
DNA (Catalog # 222902-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 222902-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L2383-FRB-GPA-NLuc was a gift from Joshua Leonard (Addgene plasmid # 222902 ; http://n2t.net/addgene:222902 ; RRID:Addgene_222902) -
For your References section:
Building Synthetic Biosensors Using Red Blood Cell Proteins. Dolberg TB, Gunnels TF, Ling T, Sarnese KA, Crispino JD, Leonard JN. ACS Synth Biol. 2024 Apr 19;13(4):1273-1289. doi: 10.1021/acssynbio.3c00754. Epub 2024 Mar 27. 10.1021/acssynbio.3c00754 PubMed 38536408
